lncRNA ANRIL play an important role in renal injury induced by diabetes

Introduction The aim of this study was to discuss the effects and mechanisms lncRNA in renal injury induced by diabetes vivo vitro study. Material methods Collecting healthy DN patients, measuring ANRIL expression RT-qPCR assay serum. Measuring normal, 10d 20d model rats kidney. LDH, MDA, SOD, IL-6 TNF-α concentration Elisa assay, evaluating kidney pathology HE staining, cell apoptosis TUNEL relative proteins IHC mRNA assay. In experiment, proliferation CCK-8, flow cytometry, WB NF-κB(p65) nuclear volume difference groups were evaluated immunofluorescence. Results Compared with people, significantly increased patients. sham group, expression, increased, SOD concentrations rate depressed day increasing. TLR4, MyD88 expressions up-regulation. Conclusions knockdown had improve via regulation TLR4/NF-κB(p65).